Display Settings:

Format

Send to:

Choose Destination
Plant Signal Behav. 2012 Aug;7(8):1027-30. doi: 10.4161/psb.20366. Epub 2012 Jul 25.

Irrepressible, truncated auxin response factors: natural roles and applications in dissecting auxin gene regulation pathways.

Author information

  • 1Department of Cell and Systems Biology, University of Toronto, Toronto, ON Canada.

Abstract

The molecularly well-characterized auxin signal transduction pathway involves two evolutionarily conserved families interacting through their C-terminal domains III and IV: the Auxin Response Factors (ARFs) and their repressors the Aux/IAAs, to control auxin-responsive genes, among them genes involved in auxin transport. ( 1) (,) ( 2) We have developed a new genetic tool to study ARF function. Using MONOPTEROS (MP)/ARF5, we have generated a truncated version of MP (MPΔ), ( 3) which has lost the target domains for repression by Aux/IAA proteins. Besides exploring genetic interactions between MP and Aux/IAAs, we used this construct to trace MP's role in vascular patterning, a previously characterized auxin dependent process. ( 4) (,) ( 5) Here we summarize examples of naturally occurring truncated ARFs and summarize potential applications of truncated ARFs as analytical tools.

PMID:
22827953
[PubMed - indexed for MEDLINE]
PMCID:
PMC3474672
Free PMC Article

Images from this publication.See all images (2)Free text

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Landes Bioscience Icon for PubMed Central
    Loading ...
    Write to the Help Desk