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J Clin Virol. 2012 Nov;55(3):209-13. doi: 10.1016/j.jcv.2012.07.001. Epub 2012 Jul 21.

Characterization of a novel gyrovirus in human stool and chicken meat.

Author information

  • 1Centre for Influenza Research and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region.

Abstract

BACKGROUND:

Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens.

OBJECTIVES:

To identify novel viruses in human stool specimens from patients with diarrhea and to investigate the ecology and clinical significance of such viruses.

STUDY DESIGN:

Nucleic acid extracted from stool specimens from patients with diarrhea with no known etiology were subjected to random PCR amplification and Roche/454 pyrosequencing. Novel viruses identified were genetically and epidemiologically characterized.

RESULTS:

Four gyroviruses, chicken anemia virus (CAV), human gyrovirus (HGV)/avian gyrovirus 2 (AGV2), gyrovirus 3 (GyV3) and a novel gyrovirus (tentatively designated as gyrovirus 4 (GyV4)) were identified in human stool specimens. GyV4, as well as CAV and AGV2/HGV were also detected in chicken skin and meat used for human consumption.

CONCLUSIONS:

A novel gyrovirus (GyV4) was identified in human stool and in chicken meat sold for human consumption. This virus was phylogenetically distinct from previously reported gyroviruses in chicken and humans (chicken anemia virus, human gyrovirus, avian gyrovirus 2 and recently reported gyrovirus 3). The epidemiology and pathogenesis of this virus in humans and in chicken needs to be further investigated.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID:
22824231
[PubMed - indexed for MEDLINE]
PMCID:
PMC3449218
Free PMC Article

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