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J Biol Eng. 2012 Jul 23;6(1):10. doi: 10.1186/1754-1611-6-10.

Temperature dependence of ssrA-tag mediated protein degradation.

Author information

  • 1MIT Synthetic Biology Center, Massachusetts Institute of Technology, 500 Technology Square, NE47-235, Cambridge, MA 02139 USA. opurcell@mit.edu.

Abstract

: Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be subjected to variable conditions, and be required to display precise, quantitative dynamics, which will be more susceptible to environmental changes than the general qualitative dynamics focussed on so far.

PMID:
22824000
[PubMed]
PMCID:
PMC3511798
Free PMC Article

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