The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors

Rong Chen; Hongling Jiang; Lin Li; Qingzhe Zhai; Linlin Qi; Wenkun Zhou; Xiaoqiang Liu; Hongmei Li; Wenguang Zheng; Jiaqiang Sun; Chuanyou Li

doi:10.1105/tpc.112.098277

The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors

Plant Cell. 2012 Jul;24(7):2898-916. doi: 10.1105/tpc.112.098277. Epub 2012 Jul 20.

Authors

Rong Chen¹, Hongling Jiang, Lin Li, Qingzhe Zhai, Linlin Qi, Wenkun Zhou, Xiaoqiang Liu, Hongmei Li, Wenguang Zheng, Jiaqiang Sun, Chuanyou Li

Affiliation

¹ State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Abstract

Transcriptional regulation plays a central role in plant hormone signaling. At the core of transcriptional regulation is the Mediator, an evolutionarily conserved, multisubunit complex that serves as a bridge between gene-specific transcription factors and the RNA polymerase machinery to regulate transcription. Here, we report the action mechanisms of the MEDIATOR25 (MED25) subunit of the Arabidopsis thaliana Mediator in regulating jasmonate- and abscisic acid (ABA)-triggered gene transcription. We show that during jasmonate signaling, MED25 physically associates with the basic helix-loop-helix transcription factor MYC2 in promoter regions of MYC2 target genes and exerts a positive effect on MYC2-regulated gene transcription. We also show that MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. Our results reveal that underlying the distinct effects of MED25 on jasmonate and ABA signaling, the interaction mechanisms of MED25 with MYC2 and ABI5 are different. These results highlight that the MED25 subunit of the Arabidopsis Mediator regulates a wide range of signaling pathways through selectively interacting with specific transcription factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / metabolism
Arabidopsis / cytology
Arabidopsis / genetics
Arabidopsis / physiology*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics*
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / metabolism
Basic-Leucine Zipper Transcription Factors / genetics*
Basic-Leucine Zipper Transcription Factors / metabolism
Cyclopentanes / metabolism
DNA-Binding Proteins
Flowers / cytology
Flowers / genetics
Flowers / physiology
Gene Expression Regulation, Plant / physiology
Germination
Mutation
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Oxylipins / metabolism
Plant Growth Regulators / metabolism*
Plant Leaves / cytology
Plant Leaves / genetics
Plant Leaves / physiology
Plant Roots / cytology
Plant Roots / genetics
Plant Roots / physiology
Plants, Genetically Modified
Promoter Regions, Genetic
Protein Interaction Mapping
Protein Structure, Tertiary
Seedlings / cytology
Seedlings / genetics
Seedlings / physiology
Seeds / cytology
Seeds / genetics
Seeds / physiology
Signal Transduction / physiology*
Time Factors

Substances

ABI5 protein, Arabidopsis
Arabidopsis Proteins
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Basic-Leucine Zipper Transcription Factors
Cyclopentanes
DNA-Binding Proteins
MYC2 protein, Arabidopsis
Nuclear Proteins
Oxylipins
PFT1 protein, Arabidopsis
Plant Growth Regulators
jasmonic acid
Abscisic Acid