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BMC Genomics. 2012 Jul 20;13:324. doi: 10.1186/1471-2164-13-324.

Composition and organization of active centromere sequences in complex genomes.

Author information

  • 1Genome Biology Group, Duke Institute for Genome Sciences & Policy, Duke University, Durham, NC, USA. kehayden@soe.ucsc.edu

Abstract

BACKGROUND:

Centromeres are sites of chromosomal spindle attachment during mitosis and meiosis. While the sequence basis for centromere identity remains a subject of considerable debate, one approach is to examine the genomic organization at these active sites that are correlated with epigenetic marks of centromere function.

RESULTS:

We have developed an approach to characterize both satellite and non-satellite centromeric sequences that are missing from current assemblies in complex genomes, using the dog genome as an example. Combining this genomic reference with an epigenetic dataset corresponding to sequences associated with the histone H3 variant centromere protein A (CENP-A), we identify active satellite sequence domains that appear to be both functionally and spatially distinct within the overall definition of satellite families.

CONCLUSIONS:

These findings establish a genomic and epigenetic foundation for exploring the functional role of centromeric sequences in the previously sequenced dog genome and provide a model for similar studies within the context of less-characterized genomes.

PMID:
22817545
[PubMed - indexed for MEDLINE]
PMCID:
PMC3422206
Free PMC Article

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