Evaluation of Clearview Exact PBP2a, a new immunochromatographic assay, for detection of low-level methicillin-resistant Staphylococcus aureus (LL-MRSA)

Claire Nonhoff; Sandrine Roisin; Marie Hallin; Olivier Denis

doi:10.1128/JCM.01829-12

Evaluation of Clearview Exact PBP2a, a new immunochromatographic assay, for detection of low-level methicillin-resistant Staphylococcus aureus (LL-MRSA)

J Clin Microbiol. 2012 Oct;50(10):3359-60. doi: 10.1128/JCM.01829-12. Epub 2012 Jul 18.

Authors

Claire Nonhoff¹, Sandrine Roisin, Marie Hallin, Olivier Denis

Affiliation

¹ Laboratoire de Référence MRSA-Staphylocoques, Service de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium. claire.nonhoff@erasme.ulb.ac.be

Abstract

We evaluated the performance of a new immunochromatographic assay (ICA), the Clearview Exact PBP2a, for rapid detection of penicillin-binding protein 2a (PBP2a) in a challenge set of Staphylococcus aureus strains showing MICs to oxacillin of ≤16 mg/liter. The sensitivity and specificity of the ICA were 96.6% and 100%, respectively.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacteriological Techniques / methods*
Chromatography, Affinity / methods*
Humans
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Microbial Sensitivity Tests
Oxacillin / pharmacology
Penicillin-Binding Proteins / analysis*
Sensitivity and Specificity
Staphylococcal Infections / microbiology*

Substances

Anti-Bacterial Agents
Penicillin-Binding Proteins
Oxacillin