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Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 May;43(3):348-51.

[The effects of activin A and inhibin A on the IVM of mice immature oocytes].

[Article in Chinese]

Author information

  • 1Reproductive Medicine Laboratory of the Second Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 610041, China.

Abstract

OBJECTIVE:

To study the effects of activin A (ACTA) and inhibin A (INHA) on the in vitro maturation (IVM) of mice immature eggs and early embryonic development.

METHODS:

1. The mice oocytes were cultured in the culture medium contained different concentration of ACTA and INHA (50 ng/mL, 100 ng/mL, 200 ng/mL) to find the optimum concentration; 2. The effects of different concentration of ACTA, INHA, ACTA+ INHA on the mature rates of mice oocytes were compared; 3. The effects of different concentration of ACTA, INHA, ACTA+INHA on the fertility rates and blastocysts formation rates of IVM mice eggs were also compared.

RESULTS:

The mature rates of mice oocytes in culture medium contained (100 ng/mL and 200 ng/mL) ACTA and INHA were significantly higher than that contained 50 ng/mL ACTA and INHA (P < 0.05); There was no difference between 100 ng/mL and 200 ng/mL groups. The mature rates, fertility rates and blastocyst rates of mice oocytes were significantly higher than those of control (P < 0.05).

CONCLUSION:

The mature rates, fertility rates and embryonic development potential after fertilization of mice oocytes were significantly promoted by ACTA, INHA. The optimum concentration of ACTA and INHA in culture medium was 100 ng/mL.

PMID:
22812235
[PubMed - indexed for MEDLINE]
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