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J Gastrointest Oncol. 2011 Dec;2(4):215-22. doi: 10.3978/j.issn.2078-6891.2011.015.

Exosome can prevent RNase from degrading microRNA in feces.

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  • 1Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa;

Abstract

BACKGROUND:

Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces.

METHODS:

RNase was added into culture media of HT-29 cells and fecal homogenates. The relative quantifications of miRNA were analyzed by real-time RT-PCR.

RESULTS:

Cellular miRNA or exosomal miRNA were protected from RNase by the cellular membrane or the exosome; meanwhile, free miRNA was degraded immediately and completely by RNase.

CONCLUSION:

The present study revealed that exosome or cellular membrane could prevent RNase from degrading miRNA inside the exosome or cells even in a dreadful condition, as in feces.

KEYWORDS:

Exosome; cancer screening; colonocyte; fecal miRNA test; miRNA

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