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Stroke. 2012 Sep;43(9):2476-82. doi: 10.1161/STROKEAHA.112.661819. Epub 2012 Jul 17.

Role of protease-activated receptor-1 in brain injury after experimental global cerebral ischemia.

Author information

  • 1Department of Neurosurgery, Room 5018 BSRB, University of Michigan, Ann Arbor, MI 48109-2200, USA.

Abstract

BACKGROUND AND PURPOSE:

Evidence suggests that the protease-activated receptor-1 (PAR-1), a thrombin receptor, mediates neuronal injury in experimental cerebral ischemia. The present study investigated whether PAR-1 plays a role in brain injury after global cerebral ischemia.

METHODS:

Adult male wild-type or PAR-1 knockout mice underwent a 20-minute bilateral common carotid artery occlusion or a sham operation. Behavior tests were performed before ischemia and 1, 2, and 3 days after bilateral common carotid artery occlusion. Mice were euthanized at different time points for thrombin activity, brain edema, Western blot analysis, and brain histology.

RESULTS:

Thrombin activity and PAR-1 expression were increased in the brain after bilateral common carotid artery occlusion. Compared with wild-type mice, PAR-1 knockout mice had less brain edema formation, neuronal death, and behavior impairment after bilateral common carotid artery occlusion. In addition, bilateral common carotid artery occlusion-induced activation of mitogen-activated protein kinases was absent in PAR-1 knockout mice.

CONCLUSIONS:

PAR-1 contributes to the brain injury induced by global cerebral ischemia, which may be related to activation of mitogen-activated protein kinases.

PMID:
22811450
[PubMed - indexed for MEDLINE]
PMCID:
PMC3429659
Free PMC Article

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