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World J Microbiol Biotechnol. 2012 Jan;28(1):105-11. doi: 10.1007/s11274-011-0797-0. Epub 2011 Jun 3.

A rapid and cost effective method in purifying small RNA.

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  • 1Infectious Disease Cluster, Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200, Kepala Batas, Penang, Malaysia.

Abstract

Purification of RNA fragments from a complex mixture is a very common technique, and requires consideration of the time, cost, purity and yield of the purified RNA fragments. This study describes the fastest method of purifying small RNA with the lowest cost possible, without compromizing the yield and purity. The technique describes the purification of small RNA from polyacrylamide gel, resulting in a good yield of small RNA with minimum experimental steps in avoiding degradation of the RNA, obviating the use of ethidium bromide and phenol-chloroform extraction, as well as siliconized glass wools to remove the polyacrylamide gel particles. The purified small RNA is suitable for a wide variety of applications such as ligation, end labelling with radio isotope, RT-PCR (Reverse Transcriptase-PCR), Northern blotting, experimental RNomics study and also Systematic Evolution of Ligands by Exponential Enrichment (SELEX).

PMID:
22806785
[PubMed - indexed for MEDLINE]
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