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J Cell Biol. 2012 Jul 23;198(2):173-83. doi: 10.1083/jcb.201112112. Epub 2012 Jul 16.

Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores.

Author information

  • 1Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, CH-8093 Zurich, Switzerland.

Abstract

During mitotic spindle assembly, Aurora B kinase is part of an error correction mechanism that detaches microtubules from kinetochores that are under low mechanical tension. During anaphase, however, kinetochore-microtubule attachments must be maintained despite a drop of tension after removal of sister chromatid cohesion. Consistent with this requirement, Aurora B relocates away from chromosomes to the central spindle at the metaphase-anaphase transition. By ribonucleic acid interference screening using a phosphorylation biosensor, we identified two PP1-targeting subunits, Sds22 and Repo-Man, which counteracted Aurora B-dependent phosphorylation of the outer kinetochore component Dsn1 during anaphase. Sds22 or Repo-Man depletion induced transient pauses during poleward chromosome movement and a high incidence of chromosome missegregation. Thus, our study identifies PP1-targeting subunits that regulate the microtubule-kinetochore interface during anaphase for faithful chromosome segregation.

PMID:
22801782
[PubMed - indexed for MEDLINE]
PMCID:
PMC3410419
Free PMC Article

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