Format

Send to:

Choose Destination
See comment in PubMed Commons below
Chromosoma. 2012 Oct;121(5):465-74. doi: 10.1007/s00412-012-0377-6. Epub 2012 Jul 13.

A TRF1-controlled common fragile site containing interstitial telomeric sequences.

Author information

  • 1Laboratory for Cell Biology and Genetics, The Rockefeller University, Box 159, 1230 York Avenue, New York, NY 10065, USA.

Abstract

Mouse telomeres have been suggested to resemble common fragile sites (CFS), showing disrupted TTAGGG fluorescent in situ hybridization signals after aphidicolin treatment. This "fragile" telomere phenotype is induced by deletion of TRF1, a shelterin protein that binds telomeric DNA and promotes efficient replication of the telomeric ds[TTAGGG]n tracts. Here we show that the chromosome-internal TTAGGG repeats present at human chromosome 2q14 form an aphidicolin-induced CFS. TRF1 binds to and stabilizes CFS 2q14 but does not affect other CFS, establishing 2q14 as the first CFS controlled by a sequence-specific DNA binding protein. The data show that telomeric DNA is inherently fragile regardless of its genomic position and imply that CFS can be caused by a specific DNA sequence.

PMID:
22790221
[PubMed - indexed for MEDLINE]
PMCID:
PMC3590843
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Write to the Help Desk