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Forensic Sci Int Genet. 2013 Jan;7(1):209-13. doi: 10.1016/j.fsigen.2012.06.013. Epub 2012 Jul 10.

PCR inhibitor removal using the NucleoSpin® DNA Clean-Up XS kit.

Author information

  • 1California Department of Justice Fresno Regional Laboratory, 5311 N. Woodrow Ave., Fresno, CA 93740, USA. korie.faber@doj.ca.gov

Abstract

Forensic evidence samples are collected from an unlimited variety of substrates, which may contain compounds known to inhibit the polymerase chain reaction (PCR). These PCR inhibitors are co-extracted with the DNA sample and can negatively affect the DNA typing results, which can range from partial to complete inhibition of the short tandem repeat (STR) PCR. One potential solution is to remove the PCR inhibitors from the extracts prior to the STR PCR with the NucleoSpin(®) DNA Clean-Up XS kit. The kit contains a NucleoSpin(®) XS silica column that has a special funnel design of thrust rings along with a very small silica membrane, which allows for sample elution in a small volume that is appropriate for use with current STR typing kits. The NucleoSpin(®) DNA Clean-Up XS kit was optimized for the best possible DNA recovery and then evaluated for its ability to remove eight commonly encountered PCR inhibitors including: bile salt, collagen, hematin, humic acid, indigo, melanin, tannic acid and urea. Each of these PCR inhibitors was effectively removed by the NucleoSpin(®) DNA Clean-Up XS kit as demonstrated by generating more complete STR profiles from the cleaned up inhibitor samples than from the raw inhibitor samples.

Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

PMID:
22784879
[PubMed - indexed for MEDLINE]
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