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Mutat Res. 2012 Oct 9;748(1-2):60-4. doi: 10.1016/j.mrgentox.2012.06.007. Epub 2012 Jul 5.

Automatic analysis of silver-stained comets by CellProfiler software.

Author information

  • 1Laboratorio de Radiobiología, Centro de Protección e Higiene de las Radiaciones, La Habana, Cuba. jorgee@cphr.edu.cu

Abstract

The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings.

© 2012 Elsevier B.V. All rights reserved.

PMID:
22771502
[PubMed - indexed for MEDLINE]
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