Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell Stem Cell. 2012 Jul 6;11(1):118-26. doi: 10.1016/j.stem.2012.03.011.

Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules.

Author information

  • 1CNRS URA 2578, Department of Developmental Biology, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France.

Erratum in

  • Cell Stem Cell. 2012 Aug 3;11(2):279.

Abstract

Regeneration of adult tissues depends on stem cells that are primed to enter a differentiation program, while remaining quiescent. How these two characteristics can be reconciled is exemplified by skeletal muscle in which the majority of quiescent satellite cells transcribe the myogenic determination gene Myf5, without activating the myogenic program. We show that Myf5 mRNA, together with microRNA-31, which regulates its translation, is sequestered in mRNP granules present in the quiescent satellite cell. In activated satellite cells, mRNP granules are dissociated, relative levels of miR-31 are reduced, and Myf5 protein accumulates, which initially requires translation, but not transcription. Conditions that promote the continued presence of mRNP granules delay the onset of myogenesis. Manipulation of miR-31 levels affects satellite cell differentiation ex vivo and muscle regeneration in vivo. We therefore propose a model in which posttranscriptional mechanisms hold quiescent stem cells poised to enter a tissue-specific differentiation program.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22770245
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk