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J Opt Soc Am A Opt Image Sci Vis. 2012 Jul 1;29(7):1277-87. doi: 10.1364/JOSAA.29.001277.

Setup for single-particle orbit tracking: artifacts and corrections.

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  • 1Experimental Physics IV and Bayreuth Institute of Macromolecular Research, University of Bayreuth, Bayreuth, Germany.


We report on an experimental setup for single-particle orbit tracking, which allows following fluorescent nanoparticles for more than 10 min with a temporal resolution of 4 ms and a dynamic position accuracy of better than 10 nm. On a model sample--20 nm sized fluorescent polymer beads in glycerol--we will illustrate how artifacts caused by unavoidable experimental shortcomings (might) obscure the experimental result and how misinterpretations can be prevented.

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