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Trends Biochem Sci. 2012 Aug;37(8):303-8. doi: 10.1016/j.tibs.2012.05.003. Epub 2012 Jun 29.

Understanding integration of α-helical membrane proteins: the next steps.

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  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605-2324, USA. reid.gilmore@umassmed.edu

Abstract

Integration of a protein into the endoplasmic reticulum (ER) membrane occurs through a series of multistep reactions that include targeting of ribosome-nascent polypeptide complexes to the ER, attachment of the ribosome to the protein translocation channel, lateral partitioning of α-helical transmembrane spans into the lipid bilayer, and folding of the lumenal, cytosolic and membrane-embedded domains of the protein. However, the molecular mechanisms and kinetics of these steps are still not entirely clear. To obtain a better understanding of the mechanism of membrane protein integration, we propose that it will be important to utilize in vivo experiments to examine the kinetics of membrane protein integration and in vitro experiments to characterize interactions between nascent membrane proteins, protein translocation factors and molecular chaperones.

Copyright © 2012 Elsevier Ltd. All rights reserved.

PMID:
22748693
[PubMed - indexed for MEDLINE]
PMCID:
PMC3557837
Free PMC Article

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