Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Lab Chip. 2012 Sep 7;12(17):3073-6. doi: 10.1039/c2lc40114b. Epub 2012 Jun 28.

Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography.

Author information

  • 1CNR-National Institute of Optics, Via Campi Flegrei, 34, I-80078, Pozzuoli (NA), Italy. melania.paturzo@ino.it

Abstract

We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics in flowing turbid media by digital holography. In fact, in flowing liquids with suspended colloidal particles, clear vision is hindered and cannot be recovered by any other microscopic imaging technique. On the contrary, using digital holography, clear imaging is possible thanks to the Doppler frequency shift experienced by the photons scattered by the flowing colloidal particles, which do not contribute to the interference process, i.e. the recorded hologram. The method is illustrated and imaging results are demonstrated for pure phase objects, i.e. biological cells in microfluidic channels.

PMID:
22740323
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Royal Society of Chemistry
    Loading ...
    Write to the Help Desk