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Mol Biosyst. 2012 Oct;8(10):2531-4. doi: 10.1039/c2mb25135c.

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.

Author information

  • 1EMBL, Structural and Computational Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Abstract

Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.

PMID:
22739670
[PubMed - indexed for MEDLINE]
PMCID:
PMC3441834
Free PMC Article

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