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Am J Bot. 2012 Jul;99(7):e268-73. doi: 10.3732/ajb.1100569. Epub 2012 Jun 25.

Development of indel markers from Citrus clementina (Rutaceae) BAC-end sequences and interspecific transferability in Citrus.

Author information

  • 1Centro de Protecci√≥n Vegetal y Biotecnolog√≠a, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain. patrick.ollitrault@cirad.fr

Abstract

PREMISE OF THE STUDY:

Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies.

METHODS AND RESULTS:

Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed.

CONCLUSIONS:

These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments.

PMID:
22733984
[PubMed - indexed for MEDLINE]
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