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Lab Chip. 2012 Aug 21;12(16):2951-8. doi: 10.1039/c2lc40373k. Epub 2012 Jun 22.

DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platform.

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  • 1University of Texas at Austin, Chemistry and Biochemistry Dept., 1 University Station, A4800, USA.

Abstract

This article describes the use of non-enzymatic nucleic acid circuits based on strand exchange reactions to detect target sequences on a paperfluidic platform. The DNA circuits that were implemented include a non-enzymatic amplifier and transduction to a fluorescent reporter; these yield an order of magnitude improvement in detection of an input nucleic acid signal. To further improve signal amplification and detection, we integrated the enzyme-free amplifier with loop-mediated isothermal amplification (LAMP). By bridging the gap between the low concentrations of LAMP amplicons and the limits of fluorescence detection, the non-enzymatic amplifier allowed us to detect as few as 1200 input templates in a paperfluidic format.

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