Nucleus-encoded mRNAs for chloroplast proteins GapA, PetA, and PsbO are trans-spliced in the flagellate Euglena gracilis irrespective of light and plastid function

J Eukaryot Microbiol. 2012 Nov-Dec;59(6):651-3. doi: 10.1111/j.1550-7408.2012.00634.x. Epub 2012 Jun 22.

Abstract

Euglena gracilis is a fresh-water flagellate possessing secondary chloroplasts of green algal origin. In contrast with organisms possessing primary plastids, mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis depend on neither light nor plastid function. However, it remains unknown, if all these mRNAs are trans-spliced and possess spliced leader sequence at the 5'-end and if trans-splicing depends on light or functional plastids. This study revealed that polyadenylated mRNAs encoding the chloroplast proteins glyceraldehyde-3-phosphate dehydrogenase (GapA), cytochrome f (PetA), and subunit O of photosystem II (PsbO) are trans-spliced irrespective of light or plastid function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chloroplast Proteins / genetics*
  • Cytochromes f / genetics
  • Euglena gracilis / genetics*
  • Euglena gracilis / metabolism
  • Euglena gracilis / radiation effects
  • Gene Expression Regulation*
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / genetics
  • Light
  • Photosystem II Protein Complex / genetics
  • Plastids / metabolism
  • Plastids / radiation effects
  • RNA Splicing*
  • RNA, Messenger / metabolism*

Substances

  • Chloroplast Proteins
  • Photosystem II Protein Complex
  • RNA, Messenger
  • Cytochromes f
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)