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Nature. 2012 Jun 28;486(7404):496-501. doi: 10.1038/nature11142.

A map of nucleosome positions in yeast at base-pair resolution.

Author information

  • 1Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA.

Abstract

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

PMID:
22722846
[PubMed - indexed for MEDLINE]
PMCID:
PMC3786739
Free PMC Article

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