Display Settings:

Format

Send to:

Choose Destination
World J Gastroenterol. 2012 Jun 14;18(22):2798-804. doi: 10.3748/wjg.v18.i22.2798.

Plasma microRNA profiles distinguish lethal injury in acetaminophen toxicity: a research study.

Author information

  • 1Department of Emergency Medicine, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, United States.

Abstract

AIM:

To investigate plasma microRNA (miRNA) profiles indicative of hepatotoxicity in the setting of lethal acetaminophen (APAP) toxicity in mice.

METHODS:

Using plasma from APAP poisoned mice, either lethally (500 mg/kg) or sublethally (150 mg/kg) dosed, we screened commercially available murine microRNA libraries (SABiosciences, Qiagen Sciences, MD) to evaluate for unique miRNA profiles between these two dosing parameters.

RESULTS:

We distinguished numerous, unique plasma miRNAs both up- and downregulated in lethally compared to sublethally dosed mice. Of note, many of the greatest up- and downregulated miRNAs, namely 574-5 p, 466 g, 466 f-3p, 375, 29 c, and 148 a, have been shown to be associated with asthma in prior studies. Interestingly, a relationship between APAP and asthma has been previously well described in the literature, with an as yet unknown mechanism of pathology. There was a statistically significant increase in alanine aminotransferase levels in the lethal compared to sublethal APAP dosing groups at the 12 h time point (P < 0.001). There was 90% mortality in the lethally compared to sublethally dosed mice at the 48 h time point (P = 0.011).

CONCLUSION:

We identified unique plasma miRNAs both up- and downregulated in APAP poisoning which are correlated to asthma development.

KEYWORDS:

Acetaminophen; Alanine aminotransferase; Drug-induced liver injury; Hepatotoxicity; Plasma microRNA

PMID:
22719188
[PubMed - indexed for MEDLINE]
PMCID:
PMC3374983
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Baishideng Publishing Group Co., Limited Icon for PubMed Central
    Loading ...
    Write to the Help Desk