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EMBO J. 2012 Aug 1;31(15):3270-81. doi: 10.1038/emboj.2012.164. Epub 2012 Jun 15.

Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release.

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  • 1Heidelberg University Biochemistry Center, Heidelberg, Germany.

Abstract

Regulated exocytosis requires that the assembly of the basic membrane fusion machinery is temporarily arrested. Synchronized membrane fusion is then caused by a specific trigger--a local rise of the Ca(2+) concentration. Using reconstituted giant unilamellar vesicles (GUVs), we have analysed the role of complexin and membrane-anchored synaptotagmin 1 in arresting and synchronizing fusion by lipid-mixing and cryo-electron microscopy. We find that they mediate the formation and consumption of docked small unilamellar vesicles (SUVs) via the following sequence of events: Synaptotagmin 1 mediates v-SNARE-SUV docking to t-SNARE-GUVs in a Ca(2+)-independent manner. Complexin blocks vesicle consumption, causing accumulation of docked vesicles. Together with synaptotagmin 1, complexin synchronizes and stimulates rapid fusion of accumulated docked vesicles in response to physiological Ca(2+) concentrations. Thus, the reconstituted assay resolves both the stimulatory and inhibitory function of complexin and mimics key aspects of synaptic vesicle fusion.

PMID:
22705946
[PubMed - indexed for MEDLINE]
PMCID:
PMC3411073
Free PMC Article

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