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Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627-31.

Transcriptional regulation of the yeast cytochrome c gene.

Abstract

DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.

PMID:
226972
[PubMed - indexed for MEDLINE]
PMCID:
PMC383885
Free PMC Article
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