Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Dev Dyn. 2012 Aug;241(8):1301-9. doi: 10.1002/dvdy.23818. Epub 2012 Jun 23.

A universal analysis tool for the detection of asymmetric signal distribution in microscopic images.

Author information

  • 1Department of Pathology, Stanford University School of Medicine, Stanford, California, USA. matis@stanford.edu

Abstract

BACKGROUND:

Polarization of tissue is achieved by asymmetric distribution of proteins and organelles within individual cells. However, existing quantitative assays to measure this asymmetry in an automated and unbiased manner suffer from significant limitations.

RESULTS:

Here, we report a new way to assess protein and organelle localization in tissue based on correlative fluorescence analysis. As a proof of principle, we successfully characterized planar cell polarity dependent asymmetry in developing Drosophila melanogaster tissues on the single cell level using fluorescence cross-correlation.

CONCLUSIONS:

Systematic modulation of signal strength and distribution show that fluorescence cross-correlation reliably detects asymmetry over a broad parameter space. The novel method described here produces robust, rapid, and unbiased measurement of biometrical properties of cell components in live tissue that is readily applicable in other model systems.

Copyright © 2012 Wiley Periodicals, Inc.

PMID:
22689329
[PubMed - indexed for MEDLINE]
PMCID:
PMC3469164
Free PMC Article

Images from this publication.See all images (4)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for John Wiley & Sons, Inc. Icon for PubMed Central
    Loading ...
    Write to the Help Desk