Geraniol synthase whose mRNA is induced by host-selective ACT-toxin in the ACT-toxin-insensitive rough lemon (Citrus jambhiri)

Hodaka Shishido; Yoko Miyamoto; Rika Ozawa; Shiduku Taniguchi; Junji Takabayashi; Kazuya Akimitsu; Kenji Gomi

doi:10.1016/j.jplph.2012.05.003

Geraniol synthase whose mRNA is induced by host-selective ACT-toxin in the ACT-toxin-insensitive rough lemon (Citrus jambhiri)

J Plant Physiol. 2012 Sep 15;169(14):1401-7. doi: 10.1016/j.jplph.2012.05.003. Epub 2012 Jun 4.

Authors

Hodaka Shishido¹, Yoko Miyamoto, Rika Ozawa, Shiduku Taniguchi, Junji Takabayashi, Kazuya Akimitsu, Kenji Gomi

Affiliation

¹ Kagawa University, Miki, Kagawa 761-0795, Japan.

PMID: 22673031
DOI: 10.1016/j.jplph.2012.05.003

Abstract

Host-selective toxins (HSTs) produced by some strains of Alternaria alternata are selectively toxic to certain cultivars of plants. However, the role of HSTs in toxin-insensitive plants is currently unknown. Here, we studied the role of ACT-toxin using an ACT-toxin producing A. alternata strain SH20 and the ACT-toxin-insensitive plant rough lemon. Induction of some defense related genes in response to SH20 were faster or stronger than in response to the ACT-toxin deficient SH20 mutant. By sequencing subtractive PCR clones obtained from mRNA of rough lemon leaves inoculated with SH20 after subtraction with that of the ACT-toxin deficient SH20 mutant, we isolated the SH20-responsive genes in rough lemon. Among the SH20-responsive genes analyzed in this study, we isolated a terpene synthase (TPS) gene, RlemTPS3. We also determined that RlemTPS3 localizes to the chloroplast and produces the monoterpene geraniol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternaria / chemistry*
Amino Acid Sequence
Citrus / drug effects
Citrus / enzymology*
Citrus / genetics*
Citrus / immunology
DNA, Complementary / genetics
Gene Expression Profiling
Gene Expression Regulation, Plant / drug effects*
Genes, Plant / genetics
Green Fluorescent Proteins / metabolism
Host-Parasite Interactions / drug effects
Host-Parasite Interactions / genetics*
Molecular Sequence Data
Mycotoxins / toxicity*
Phosphoric Monoester Hydrolases / chemistry
Phosphoric Monoester Hydrolases / genetics*
Phosphoric Monoester Hydrolases / metabolism
Plant Leaves / drug effects
Plant Leaves / genetics
Protein Transport / drug effects
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reproducibility of Results
Subcellular Fractions / drug effects
Subcellular Fractions / metabolism

Substances

DNA, Complementary
Mycotoxins
RNA, Messenger
Green Fluorescent Proteins
Phosphoric Monoester Hydrolases

Associated data

GENBANK/AB691531