Display Settings:

Format

Send to:

Choose Destination
Gene. 1990 Nov 30;96(1):23-8.

High efficiency transformation of Escherichia coli with plasmids.

Author information

  • 1Research Department, Turuga Enzyme Plant, Toyobo Co, Ltd., Fukui-ken, Japan.

Abstract

We have re-evaluated the conditions for preparing competent Escherichia coli cells and established a simple and efficient method (SEM) for plasmid transfection. Cells (DH5, JM109 and HB101) prepared by SEM are extremely competent for transformation (1-3 x 10(9) cfu/microgram of pBR322 DNA), and can be stored in liquid nitrogen for at least 40 days without loss of competence. Unlike electroporation, transformation using these competent cells is affected minimally by salts in DNA preparation. These competent cells are particularly useful for construction of high-complexity cDNA libraries with a minimum expenditure of mRNA.

PMID:
2265755
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk