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Genetics. 2012 Aug;191(4):1381-6. doi: 10.1534/genetics.112.141341. Epub 2012 May 29.

SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.

Author information

  • 1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zürich, Switzerland.

Abstract

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.

PMID:
22649081
[PubMed - indexed for MEDLINE]
PMCID:
PMC3416015
Free PMC Article

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