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Curr Microbiol. 2012 Sep;65(3):225-30. doi: 10.1007/s00284-012-0147-y. Epub 2012 May 29.

Biodegradation kinetics of phenanthrene by a fusant strain.

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  • 1College of Environmental Science and Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China. lujing19822@163.com

Abstract

The fusant strain (F14), which produced by protoplast fusion between Sphingomonas sp. GY2B (GenBank DQ139343) and Pseudomonas sp. GP3A (GenBank EU233280), was tested for phenanthrene biodegradation at 30 °C and pH of 7.0. The kinetics of phenanthrene biodegradation by F14 was investigated over a wide range of initial concentration (15-1,000 mg l(-1)). The rate and the extent of phenanthrene degradation increased with the increase of concentration up to 230 mg l(-1), which indicated negligible inhibition effect at low concentrations. The non-competitive inhibition model was found to be fit for the process. GC-MS analysis showed that biodegradation of phenanthrene by F14 was via dioxygenation at both 1,2- and 3,4-positions and followed by 2-hydroxy-1-naphthoic acid and 1-hydroxy-2-naphthoic acid. The relative intensity of 2-hydroxy-1-naphthoic acid was approximately 3-4 times higher than that of 1-hydroxy-2-naphthoic acid, indicating the 2-hydroxy-1-naphthoic acid was the predominant product in the phenanthrene degradation by fusant strain F14.

PMID:
22645013
[PubMed - indexed for MEDLINE]
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