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Biochimie. 2012 Sep;94(9):2058-61. doi: 10.1016/j.biochi.2012.05.014. Epub 2012 May 22.

Snap denaturation reveals dimerization by AraC-like protein Rns.

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  • 1Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.

Abstract

Here we show that the Rns regulator of Escherichia coli dimerises in vivo and in vitro. Furthermore, we demonstrate that Rns forms aggregates in vitro and describe a methodology to ameliorate aggregation thus permitting the analysis of Rns by cross-linking.

Copyright © 2012 Elsevier Masson SAS. All rights reserved.

PMID:
22627379
[PubMed - indexed for MEDLINE]
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