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Daru. 2011;19(5):326-31.

Effects of polygonum aviculare herbal extract on proliferation and apoptotic gene expression of MCF-7.

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  • 1Research center, Iranian Blood Transfusion Organization, Tehran.

Abstract

BACKGROUND AND THE PURPOSE OF THE STUDY:

One of the most common malignancies in women is breast cancer. Although several treatments for breast cancer are available, application of herbal medicine as a supplementary treatment is a new option to help curing the disease. In this study anticancer effects of Polygonum avicular herbal extract was investigated.

METHODS:

Polygonum avicular extract was obtained by methanol. MCF-7 cell line was treated with different concentrations of Polygonum avicular (50, 100, 150, 200, 250, 300,350 400 ng/µl) for different time lengths ( 6, 12, 24, and 48 hrs). MTT assay and Flow Cytometry were used to evaluate cell proliferation and apoptosis, respectively. RT-PCR was also carried out to evaluate the expression of apoptotic genes.

RESULTS AND DISCUSSION:

Results showed that Polygonum avicular induced cytotoxicity in MCF-7 cell line at concentrations higher than 300 ng/µl and this was confirmed by the highest rate of cell death as measured by Trypan Blue and MTT assays. RT-PCR results showed up-regulation of P53 and down-regulation of Bcl-2 proteins which implied the ability of Polygonum avicular to induce apoptosis in MCF-7 cells and confirmed its anticancer property. Further studies are required to evaluate effects of the extract on other apoptotic genes.

KEYWORDS:

Breast cancer; Herbal medicine; cell death; knotweed

PMID:
22615677
[PubMed]
PMCID:
PMC3304393
Free PMC Article
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