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Blood. 2012 Jul 5;120(1):130-42. doi: 10.1182/blood-2011-11-394072. Epub 2012 May 21.

Eri1 regulates microRNA homeostasis and mouse lymphocyte development and antiviral function.

Author information

  • 1Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

Abstract

Natural killer (NK) cells play a critical role in early host defense to infected and transformed cells. Here, we show that mice deficient in Eri1, a conserved 3'-to-5' exoribonuclease that represses RNA interference, have a cell-intrinsic defect in NK-cell development and maturation. Eri1(-/-) NK cells displayed delayed acquisition of Ly49 receptors in the bone marrow (BM) and a selective reduction in Ly49D and Ly49H activating receptors in the periphery. Eri1 was required for immune-mediated control of mouse CMV (MCMV) infection. Ly49H(+) NK cells deficient in Eri1 failed to expand efficiently during MCMV infection, and virus-specific responses were also diminished among Eri1(-/-) T cells. We identified miRNAs as the major endogenous small RNA target of Eri1 in mouse lymphocytes. Both NK and T cells deficient in Eri1 displayed a global, sequence-independent increase in miRNA abundance. Ectopic Eri1 expression rescued defective miRNA expression in mature Eri1(-/-) T cells. Thus, mouse Eri1 regulates miRNA homeostasis in lymphocytes and is required for normal NK-cell development and antiviral immunity.

PMID:
22613798
[PubMed - indexed for MEDLINE]
PMCID:
PMC3390952
Free PMC Article

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