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Virology. 2012 Sep 1;430(2):90-9. doi: 10.1016/j.virol.2012.04.013. Epub 2012 May 18.

Characterization of a recombinant canine coronavirus with a distinct receptor-binding (S1) domain.

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  • 1Department of Microbiology and Immunology, Veterinary Medical Center, Cornell University, Ithaca, NY 14853, United States.


Canine alphacoronaviruses (CCoV) exist in two serotypes, type I and II, both of which can cause severe gastroenteritis. Here, we characterize a canine alphacoronavirus, designated CCoV-A76, first isolated in 1976. Serological studies show that CCoV-A76 is distinct from other CCoVs, such as the prototype CCoV-1-71. Efficient replication of CCoV-A76 is restricted to canine cell lines, in contrast to the prototypical type II strain CCoV-1-71 that more efficiently replicates in feline cells. CCoV-A76 can use canine aminopeptidase N (cAPN) receptor for infection of cells, but was unable to use feline APN (fAPN). In contrast, CCoV-1-71 can utilize both. Genomic analysis shows that CCoV-A76 possesses a distinct spike, which is the result of a recombination between type I and type II CCoV, that occurred between the N- and C-terminal domains (NTD and C-domain) of the S1 subunit. These data suggest that CCoV-A76 represents a recombinant coronavirus form, with distinct host cell tropism.

Copyright © 2012 Elsevier Inc. All rights reserved.

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