Abstract
Northern blotting is a valuable method for detection and quantification of RNA in the field of virology. Although many methods including a various versions of polymerase chain reaction have been developed over the years, Northern blotting has been still considered as a useful and effective method for the analysis of progeny RNA accumulation for viral and subviral pathogens, such as satellite RNAs, in plant hosts. Here, we describe a detailed Northern blot protocol for efficient detection and quantification of viral and satellite RNAs from plant hosts.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Blotting, Northern / methods*
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Bromovirus / genetics*
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Buffers
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Cucumber Mosaic Virus Satellite / genetics*
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Cucumovirus / genetics*
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Densitometry
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Electrophoresis, Agar Gel / methods
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Formaldehyde / chemistry
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Nucleic Acid Denaturation
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Nucleic Acid Hybridization / methods
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Phosphorus Radioisotopes
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Plants / virology*
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RNA, Viral / genetics
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RNA, Viral / isolation & purification*
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Staining and Labeling
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Transcription, Genetic
Substances
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Buffers
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Cucumber Mosaic Virus Satellite
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Phosphorus Radioisotopes
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RNA, Viral
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Formaldehyde