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Nat Methods. 2012 Jun;9(6):594-6. doi: 10.1038/nmeth.2017. Epub 2012 May 13.

M-Track: detecting short-lived protein-protein interactions in vivo.

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  • 1Department of Biochemistry and Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, Austria.

Abstract

We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.

PMID:
22581371
[PubMed - indexed for MEDLINE]
PMCID:
PMC3428623
Free PMC Article

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