Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Biochem Parasitol. 2012 Jul;184(1):59-62. doi: 10.1016/j.molbiopara.2012.04.007. Epub 2012 Apr 24.

Transient and stable transfection in the protozoan parasite Entamoeba invadens.

Author information

  • 1Division of Infectious Diseases, Department of Internal Medicine, Stanford University, Stanford, CA 94305-5107, USA.

Abstract

Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID:
22561071
[PubMed - indexed for MEDLINE]
PMCID:
PMC3358517
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science Icon for PubMed Central Icon for Faculty of 1000
    Loading ...
    Write to the Help Desk