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Vaccine. 2012 Jun 29;30(31):4581-4. doi: 10.1016/j.vaccine.2012.04.059. Epub 2012 Apr 30.

Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

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  • 1David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA.

Abstract

Although previous studies have found minimal changes in CD4 T cell responses after vaccination of adults with trivalent inactivated influenza vaccine, daily sampling and monitoring of the proliferation marker Ki-67 have now been used to reveal that a substantial fraction of influenza-specific CD4 T cells respond to vaccination. At 4-6 days after vaccination, there is a sharp rise in the numbers of Ki-67-expressing PBMC that produce IFNγ, IL-2 and/or TNFα in vitro in response to influenza vaccine or peptide. Ki-67(+) cell numbers then decline rapidly, and 10 days after vaccination, both Ki-67(+) and overall influenza-specific cell numbers are similar to pre-vaccination levels. These results provide a tool for assessing the quality and quantity of CD4 T cell responses to different influenza vaccines, and raise the possibility that the anti-influenza T cell memory response may be qualitatively altered by vaccination, even if the overall memory cell numbers do not change significantly.

Copyright © 2012. Published by Elsevier Ltd.

PMID:
22554464
[PubMed - indexed for MEDLINE]
PMCID:
PMC3858959
Free PMC Article
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