Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nanotoxicology. 2013 Aug;7(5):963-73. doi: 10.3109/17435390.2012.689880. Epub 2012 Jul 27.

Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles.

Author information

  • 1European Centre of Environment and Human Health, Peninsula College of Medicine and Dentistry, University of Exeter , Truro, Cornwall, TR1 3HD , UK. michaela_kendall@yahoo.co.uk

Abstract

Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti-inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measured NP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). SP-D aggregated NPs, especially following the addition of calcium. Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. rfhSP-D enhanced the co-localisation of NPs to epithelial A549 cells in vitro. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.

PMID:
22551051
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Informa Healthcare
    Loading ...
    Write to the Help Desk