Abstract
We have developed a novel method for the expression and purification of p27, the major core protein of simian immunodeficiency virus. Circular dichroism measurements of purified p27 were used to determine the relative amounts of alpha-helix, beta-sheet and unordered secondary structural elements. These empirically determined values appear to be inconsistent with previously published theoretical models based on homology comparisons.
MeSH terms
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Circular Dichroism
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Cloning, Molecular
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Gene Products, gag / chemistry
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Gene Products, gag / genetics
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Gene Products, gag / isolation & purification*
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Plasmids
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Protein Conformation
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Simian Immunodeficiency Virus / analysis*
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Simian Immunodeficiency Virus / genetics
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Viral Core Proteins / isolation & purification*
Substances
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Gag protein p27, Simian immunodeficiency virus
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Gene Products, gag
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Viral Core Proteins