Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus)

Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):691-695. doi: 10.1099/ijs.0.040931-0. Epub 2012 Apr 27.

Abstract

An obligately anaerobic, non-pigmented, non-spore-forming, Gram-staining-negative, rod-shaped bacterium, designated strain Rs-03(T), was isolated from the gut of the subterranean termite Reticulitermes speratus. The taxonomic position of the novel strain was determined by following a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Rs-03(T) was a member of the genus Bacteroides and was most closely related to Bacteroides thetaiotaomicron JCM 5827(T) (95.0 % sequence similarity), Bacteroides faecis JCM 16478(T) (94.8 %) and Bacteroides xylanisolvens JCM 15633(T) (94.3 %). The results of hsp60 gene sequence analysis indicated that the novel strain was different from established members of the genus Bacteroides. Strain Rs-03(T) was saccharolytic and produced succinic and acetic acids, with small amounts of propionic acid, as metabolic end products. The major cellular fatty acids of strain Rs-03(T) were anteiso-C(15 : 0), C(18 : 1)ω9c and iso-C(17 : 0) 3-OH. The major menaquinones were MK-10 and MK-9 and the genomic DNA G+C content was 44.9 mol%. Based on these data, strain Rs-03(T) represents a novel species in the genus Bacteroides, for which the name Bacteroides reticulotermitis sp. nov. is proposed. The type strain is Rs-03(T) ( = JCM 10512(T) = CCUG 62153(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Bacteroides / classification*
  • Bacteroides / genetics
  • Bacteroides / isolation & purification
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Gastrointestinal Tract / microbiology*
  • Isoptera / microbiology*
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2

Associated data

  • GENBANK/AB692943
  • GENBANK/AB692944