Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis

J Immunol Methods. 2012 Jul 31;381(1-2):32-40. doi: 10.1016/j.jim.2012.04.006. Epub 2012 Apr 20.

Abstract

The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1 mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology*
  • Chorionic Gonadotropin, beta Subunit, Human / analysis
  • Chorionic Gonadotropin, beta Subunit, Human / immunology*
  • Female
  • Humans
  • Immunoassay / methods*
  • Magnetite Nanoparticles / chemistry*
  • Pregnancy
  • Pregnancy Tests / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Chorionic Gonadotropin, beta Subunit, Human
  • Magnetite Nanoparticles