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Mol Cell. 2012 May 25;46(4):449-60. doi: 10.1016/j.molcel.2012.03.023. Epub 2012 Apr 26.

Nitric oxide modulates bacterial biofilm formation through a multicomponent cyclic-di-GMP signaling network.

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  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Abstract

Nitric oxide (NO) signaling in vertebrates is well characterized and involves the heme-nitric oxide/oxygen-binding (H-NOX) domain of soluble guanylate cyclase as a selective NO sensor. In contrast, little is known about the biological role or signaling output of bacterial H-NOX proteins. Here, we describe a molecular pathway for H-NOX signaling in Shewanella oneidensis. NO stimulates biofilm formation by controlling the levels of the bacterial secondary messenger cyclic diguanosine monophosphate (c-di-GMP). Phosphotransfer profiling was used to map the connectivity of a multicomponent signaling network that involves integration from two histidine kinases and branching to three response regulators. A feed-forward loop between response regulators with phosphodiesterase domains and phosphorylation-mediated activation intricately regulated c-di-GMP levels. Phenotypic characterization established a link between NO signaling and biofilm formation. Cellular adhesion may provide a protection mechanism for bacteria against reactive and damaging NO. These results are broadly applicable to H-NOX-mediated NO signaling in bacteria.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22542454
[PubMed - indexed for MEDLINE]
PMCID:
PMC3361614
Free PMC Article

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