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Front Microbiol. 2012 Apr 23;3:155. doi: 10.3389/fmicb.2012.00155. eCollection 2012.

Effect of Metals on the Lytic Cycle of the Coccolithovirus, EhV86.

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  • 1School of Ocean and Earth Science, University of Southampton, National Oceanography Centre Southampton, UK.

Abstract

In this study we show that metals, and in particular copper (Cu), can disrupt the lytic cycle in the Emiliania huxleyi - EhV86 host-virus system. E. huxleyi lysis rates were reduced at high total Cu concentrations (> approximately 500 nM) in the presence and absence of EDTA (ethylenediaminetetraacetic acid) in acute short term exposure experiments. Zinc (Zn), cadmium (Cd), and cobalt (Co) were not observed to affect the lysis rate of EhV86 in these experiments. The cellular glutathione (GSH) content increased in virus infected cells, but not as a result of metal exposure. In contrast, the cellular content of phytochelatins (PCs) increased only in response to metal exposure. The increase in glutathione content is consistent with increases in the production of reactive oxygen species (ROS) on viral lysis, while increases in PC content are likely linked to metal homeostasis and indicate that metal toxicity to the host was not affected by viral infection. We propose that Cu prevents lytic production of EhV86 by interfering with virus DNA (deoxyribonucleic acid) synthesis through a transcriptional block, which ultimately suppresses the formation of ROS.

KEYWORDS:

Emiliania huxleyi; cadmium; copper; glutathione; phytochelatins; phytoplankton; thiols; virus

PMID:
22536202
[PubMed]
PMCID:
PMC3333479
Free PMC Article
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