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J Clin Microbiol. 2012 Jul;50(7):2503-5. doi: 10.1128/JCM.00479-12. Epub 2012 Apr 25.

Comparison of medium, temperature, and length of incubation for detection of vancomycin-resistant Enterococcus.

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  • 1Division of Medical Microbiology, Department of Pathology and Laboratory Medicine, University of California, Irvine, Medical Center, Orange, California, USA.

Abstract

Campylobacter (Campy; BD Diagnostics, Sparks, MD), Spectra VRE (Remel, Lenexa, KS), and bile-esculin-azide-vancomycin (BEAV; Remel) agars were compared for their ability to detect vancomycin-resistant enterococci (VRE) in 750 stool specimens. The media were compared at 24 h and 48 h of incubation at 35°C and 42°C. When incubated for 24 h at 35°C, Campy was the most sensitive (97.8%) and specific (99.9%) but was comparable to Spectra, which has a sensitivity of 95.6% and a specificity of 99.1%, whereas BEAV was significantly less sensitive (90%) and specific (96.1%). Incubation at 42°C or extended incubation at 35°C for 48 h yielded no advantage over incubation at 35°C for 24 h.

PMID:
22535989
[PubMed - indexed for MEDLINE]
PMCID:
PMC3405587
Free PMC Article
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