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Nucleic Acids Res. 1990 Nov 25;18(22):6559-64.

Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.

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  • 1Institute of Cell and Molecular Biology, University of Edinburgh, UK.


PRP2 protein of Saccharomyces cerevisiae is required for the pre-mRNA splicing reaction but not for the early stages of spliceosome assembly. Using anti-PRP2 antibodies we demonstrate that PRP2 protein is associated with spliceosomes prior to, and throughout step 1 of the splicing reaction. Heat-inactivated prp2 protein, by contrast, does not seem to associate with spliceosomes. By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly.

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