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J Physiol. 2012 Jul 1;590(Pt 13):2955-63. doi: 10.1113/jphysiol.2012.228114. Epub 2012 Apr 16.

Reduction of motion artifacts during in vivo two-photon imaging of brain through heartbeat triggered scanning.

Author information

  • 1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 N. Wolfe St, WBSB 1001, Baltimore, MD 21205, USA. martin.paukert.01@gmail.com

Abstract

Two-photon imaging of fluorescence in brain enables analysis of the structure and dynamic activity of neurons and glial cells in living animals. However, vital functions such as beating of the heart cause pulsations in brain tissue, leading to image distortion and loss of resolution. We find that synchronizing imaging scans to the cardiac cycle reduces motion artifacts, significantly improving the resolution of cellular structures. By interlacing multiple heartbeat triggered imaging scans, it was possible to image large brain volumes with negligible distortion. This approach can be readily incorporated into conventional microscopes to achieve substantial reductions in motion artifacts during two-photon imaging.

PMID:
22508962
[PubMed - indexed for MEDLINE]
PMCID:
PMC3406383
Free PMC Article

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