Nat Genet. 2012 Apr 15;44(5):552-61. doi: 10.1038/ng.2250.
Identification of common variants associated with human hippocampal and intracranial volumes.
Stein JL,
Medland SE,
Vasquez AA,
Hibar DP,
Senstad RE,
Winkler AM,
Toro R,
Appel K,
Bartecek R,
Bergmann Ø,
Bernard M,
Brown AA,
Cannon DM,
Chakravarty MM,
Christoforou A,
Domin M,
Grimm O,
Hollinshead M,
Holmes AJ,
Homuth G,
Hottenga JJ,
Langan C,
Lopez LM,
Hansell NK,
Hwang KS,
Kim S,
Laje G,
Lee PH,
Liu X,
Loth E,
Lourdusamy A,
Mattingsdal M,
Mohnke S,
Maniega SM,
Nho K,
Nugent AC,
O'Brien C,
Papmeyer M,
Pütz B,
Ramasamy A,
Rasmussen J,
Rijpkema M,
Risacher SL,
Roddey JC,
Rose EJ,
Ryten M,
Shen L,
Sprooten E,
Strengman E,
Teumer A,
Trabzuni D,
Turner J,
van Eijk K,
van Erp TG,
van Tol MJ,
Wittfeld K,
Wolf C,
Woudstra S,
Aleman A,
Alhusaini S,
Almasy L,
Binder EB,
Brohawn DG,
Cantor RM,
Carless MA,
Corvin A,
Czisch M,
Curran JE,
Davies G,
de Almeida MA,
Delanty N,
Depondt C,
Duggirala R,
Dyer TD,
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Fagerness J,
Fox PT,
Freimer NB,
Gill M,
Göring HH,
Hagler DJ,
Hoehn D,
Holsboer F,
Hoogman M,
Hosten N,
Jahanshad N,
Johnson MP,
Kasperaviciute D,
Kent JW Jr,
Kochunov P,
Lancaster JL,
Lawrie SM,
Liewald DC,
Mandl R,
Matarin M,
Mattheisen M,
Meisenzahl E,
Melle I,
Moses EK,
Mühleisen TW,
Nauck M,
Nöthen MM,
Olvera RL,
Pandolfo M,
Pike GB,
Puls R,
Reinvang I,
Rentería ME,
Rietschel M,
Roffman JL,
Royle NA,
Rujescu D,
Savitz J,
Schnack HG,
Schnell K,
Seiferth N,
Smith C,
Steen VM,
Valdés Hernández MC,
Van den Heuvel M,
van der Wee NJ,
Van Haren NE,
Veltman JA,
Völzke H,
Walker R,
Westlye LT,
Whelan CD,
Agartz I,
Boomsma DI,
Cavalleri GL,
Dale AM,
Djurovic S,
Drevets WC,
Hagoort P,
Hall J,
Heinz A,
Jack CR Jr,
Foroud TM,
Le Hellard S,
Macciardi F,
Montgomery GW,
Poline JB,
Porteous DJ,
Sisodiya SM,
Starr JM,
Sussmann J,
Toga AW,
Veltman DJ,
Walter H,
Weiner MW;
Alzheimer's Disease Neuroimaging Initiative;
EPIGEN Consortium;
IMAGEN Consortium;
Saguenay Youth Study Group,
Bis JC,
Ikram MA,
Smith AV,
Gudnason V,
Tzourio C,
Vernooij MW,
Launer LJ,
DeCarli C,
Seshadri S;
Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium,
Andreassen OA,
Apostolova LG,
Bastin ME,
Blangero J,
Brunner HG,
Buckner RL,
Cichon S,
Coppola G,
de Zubicaray GI,
Deary IJ,
Donohoe G,
de Geus EJ,
Espeseth T,
Fernández G,
Glahn DC,
Grabe HJ,
Hardy J,
Hulshoff Pol HE,
Jenkinson M,
Kahn RS,
McDonald C,
McIntosh AM,
McMahon FJ,
McMahon KL,
Meyer-Lindenberg A,
Morris DW,
Müller-Myhsok B,
Nichols TE,
Ophoff RA,
Paus T,
Pausova Z,
Penninx BW,
Potkin SG,
Sämann PG,
Saykin AJ,
Schumann G,
Smoller JW,
Wardlaw JM,
Weale ME,
Martin NG,
Franke B,
Wright MJ,
Thompson PM;
Enhancing Neuro Imaging Genetics through Meta-Analysis Consortium.
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Source
Laboratory of Neuro Imaging, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Abstract
Identifying genetic variants influencing human brain structures may reveal new biological mechanisms underlying cognition and neuropsychiatric illness. The volume of the hippocampus is a biomarker of incipient Alzheimer's disease and is reduced in schizophrenia, major depression and mesial temporal lobe epilepsy. Whereas many brain imaging phenotypes are highly heritable, identifying and replicating genetic influences has been difficult, as small effects and the high costs of magnetic resonance imaging (MRI) have led to underpowered studies. Here we report genome-wide association meta-analyses and replication for mean bilateral hippocampal, total brain and intracranial volumes from a large multinational consortium. The intergenic variant rs7294919 was associated with hippocampal volume (12q24.22; N = 21,151; P = 6.70 × 10(-16)) and the expression levels of the positional candidate gene TESC in brain tissue. Additionally, rs10784502, located within HMGA2, was associated with intracranial volume (12q14.3; N = 15,782; P = 1.12 × 10(-12)). We also identified a suggestive association with total brain volume at rs10494373 within DDR2 (1q23.3; N = 6,500; P = 5.81 × 10(-7)).
- PMID:
- 22504417
- [PubMed - indexed for MEDLINE]
- PMCID:
- PMC3635491
Free PMC ArticleFigure 1
Association results and meta-analysis of effects in individual and combined analyses. (a) The strongest association with hippocampal volume was found for rs7294919. Fixed-effects meta-analysis P values are shown41 after controlling for intracranial volume using all subjects in the discovery sample. (b) The strongest association with intracranial volume was found for rs10784502. Fixed-effects meta-analysis P values are shown in healthy subjects only. (c,d) The effect within each sample contributing to the meta-analysis is shown in forest plots for hippocampal volume (c) and intracranial volume (d). Association data using intracranial volume as a phenotype were not available for the EPIGEN sample. Head size was not controlled for in the CHARGE Consortium association analyses.
Nat Genet. 2012 April 15;44(5):552-561.
Figure 2
Association of rs7294919 with hippocampal volume stratified by disease and covariates. Effects are consistent in the discovery sample regardless of whether individuals with disease (N = 7,795) or only healthy subjects (N = 5,775) were included. The effect is also consistent whether accounting for intracranial volume (ICV), total brain volume (TBV) or without a measure of head size (Other).
Nat Genet. 2012 April 15;44(5):552-561.
Figure 3
Regulatory effects of hippocampal-associated variant and expression of TESC within the hippocampus. (a–d) The locus most associated with hippocampal volume was also associated with mRNA expression of the TESC gene in brain in three independent samples, the UCL epilepsy cohort (a), the SNPExpress database (b), where a proxy SNP was used, and the UK Brain Expression Database (d), where differences in TESC expression of the directly genotyped hippocampal variant (rs7294919) were strongest in the temporal cortex (TCTX) (red box) but also found in the average expression of all cortex (AvgCTX) and average expression of all brain structures tested (AvgALL). Symbol color represents genotype in a and d. These regional gradients in expression support the hypothesis that the SNP may associate with hippocampal but not total brain volume. No effects were detected in PBMCs from the SNPExpress database (c). CRBL, cerebellar cortex; FCTX, frontal cortex; HIPP, hippocampus; MEDU, medulla (specifically the inferior olivary nucleus); OCTX, occipital cortex; PUTM, putamen; SNIG, substantia nigra; THAL, thalamus; WHMT, intralobular white matter. (e) TESC is differentially expressed within the fetal human brain (P = 1.33 × 10−12), with the highest expression in striatum (STRIAT) and hippocampus (HIPP)42. Box plots represent median and 25th to 75th percentiles. Upper and lower lines show minimum and maximum values, respectively. CBLM, cerebellum; DLPFC, dorsolateral prefrontal neocortex; MPFC, medial prefrontal neocortex; MS, motor-somatosensory neocortex; OCC, occipital visual neocortex; OFC, orbital prefrontal neocortex; PAS, parietal association neocortex; TAC, temporal association neocortex; TAU, temporal auditory neocortex; THAL, mediodorsal thalamus; VLPFC, ventrolateral prefrontal neocortex. (f) TESC has moderate to high gene expression throughout the adult human hippocampus (shown in green), as visualized in the Allen Institute Human Brain Atlas using Brain Explorer 2 software. An inferior view of the brain is shown in two subjects; the anterior portion of the brain is at the top. The colors of spheres within the hippocampus indicate the Z-scores of TESC expression normalized within each subject across brain structures. Heat maps show that expression of TESC is higher in the hippocampus (HiF) and striatum (Str) than in other brain structures.
Nat Genet. 2012 April 15;44(5):552-561.
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