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Chin Med J (Engl). 2012 Feb;125(4):617-21.

E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells.

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  • 1Department of Respiratory Medicine, Second Military Medical University/Nanjing General Hospital of Nanjing Military Command, People's Liberation Army, Nanjing, Jiangsu, China.

Abstract

BACKGROUND:

Aspergillus fumigatus (A. fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy, organ transplantation or with persistent neutropenia. This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A. fumigatus blastospores in the human epithelial cell line A549.

METHODS:

A. fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure. After establishing the adhesion model, the adhesion and endocytosis of A. fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA (siRNA).

RESULTS:

E-cadherin was adhered to the surface of A. fumigatus blastospore. Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells.

CONCLUSIONS:

E-cadherin is a receptor for adhesion and endocytosis of A. fumigatus blastospores in epithelial cells. This may open a new approach to treat this fungal infection.

PMID:
22490484
[PubMed - indexed for MEDLINE]
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